β-榄香烯诱导小鼠肝癌细胞早期凋亡

Early apoptosis of mice hepatoma HcaF cells induced by β-elemene

目的观察 β 榄香烯诱导小鼠肝癌HcaF细胞早期凋亡的作用。方法Annexin V FITC标记后FCM分析细胞早期凋亡。结果 5 0 μg/mlβ 榄香烯作用 2h可诱导HcaF细胞早期凋亡 ,而大剂量 (10 0～ 2 0 0 μg/ml)则呈现明显细胞毒作用。 结论 β 榄香烯诱导小鼠肝癌细胞早期凋亡呈剂量依赖性 ;Annexin

Objective To observe the effect of β-elemene on early apoptosis of hepatoma cells(HcaF) in mice. Methods Flow cytometry was used to analyse the early apoptosis of HcaF cells labelled with annexin-V-FITC. Results β-Elemene could induce the early apoptosis of HcaF cells after treatment (50μg/ml) for 2 hours, while it had a significant cytotoxic effect on HcaF cells at high concentration of 100-200μg/ml. Conclusions β-Elemene could induce the early apoptosis of HcaF cells which was associated with concentration of the drug. The annexin-V-FITC labeling is a sensitive and specific marker for evaluating the early apoptosis of cells.