冠状动脉粥样硬化性心脏病患者载脂蛋白(a)五核苷酸重复序列基因多态性的初步研究

A preliminary study of pentanucleotide repeat polymorphism of apolipoprotein(a) gene in coronary atherosclerotic heart disease

目的 探讨载脂蛋白 (a) [apolipoprotein(a) ,apo(a) ]五核苷酸重复序列 (pentanucleotiderepeats,PNR)基因多态性与中国汉族人冠状动脉粥样硬化性心脏病 (简称冠心病 )发病的关系。方法 应用聚合酶链反应结合聚丙烯酰胺凝胶电泳和硝酸银染色 ,对 15 3名中国汉族正常人和 16 5例冠心病患者apo(a) PNR基因多态性进行了分析。结果 冠心病组 apo(a) PNR(TTTTA) 5/8基因型频率 (0 .188)和(TTTTA) 5等位基因频率 (0 .115 )显著高于正常对照组 (0 .0 39,0 .0 2 6 ) ,差异有显著性 (P <0 .0 1,P<0 .0 5 )。结论 apo(a) PNR基因多态性与人群易患冠心病有关 ,可能在一定程度上参与了冠心病的发生和发展过程。

Objective. This study inquired into the relationship between a pentanucleotide repeats (PNR) polymorphism of the apolipoprotein (a) [apo (a)] gene and coronary atherosclerotic heart disease (CHD) in Chinese Han nationality. Methods. PNR polymorphism of the apo(a) gene from 165 cases of CHD and 153 normal individuals were analyzed by polymerase chain reaction (PCR) -denature polyacrylamide gel electrophoresis-silver stain. Results. The frequencies of (TTTTA)5/8 genotype (0. 188) and (TTTTA)5 allele (0. 115) in CHD group were remarkably higher than those in control group (0. 039, 0. 026) (P<0.01,P<0.05). Conclusion. These findings indicate that the PNR polymorphism of apo(a) is associated with the susceptibility to CHD, which may be involved in the development of CHD.