D16S539、D7S820和D13S317位点在新疆哈萨克族中的遗传多态性

Genetic polymorphism of D16S539,D7S820 and D13S317 loci in the Kazak ethnic population

目的 了解新疆哈萨克族人群 D16 S5 39、D7S82 0、D13S3173个 STR位点的遗传多态性。方法 应用多重 PCR扩增 ,6 %变性聚丙烯酰胺凝胶电泳结合银染技术对 10 2名无关个体及 8个家系 42人的哈萨克族人群进行调查 ,并与其他人群进行了比较。结果 3个位点分别检测出 8、7、8个等位片段 ,多态性分布符合 Hardy- Weinberg平衡定律。期望杂合度为 :0 .9439、0 .935 6、0 .930 4,累积多态信息量为0 .990 5、个体识别为 0 .9998、非父排除率为 0 .95 72 ,与其他人群比较差异有显著性 (P<0 .0 5 ) ,在家系调查中无一突变发现 ,均按孟德尔遗传规律传递。结论 3个 STR位点的综合检验在法医学应用及群体遗传学中显示了较高的价值。

Objective To analyze the genetic polymorphism of D16S539, D7S820 and D13S317 in Chinese Kazak ethnic population from Xinjiang. Methods One hundred and two unrelated individuals and a sample of families ( n =42) were investigated by multiplex amplification, 6% denaturing PAGE and silver staining. And, the obtained allele frequencies were compared with those of other populations. Results Eight, seven, eight alleles were observed at the 3 STR loci respectively and the genotypes distributions were in accordance with Hardy-Weinberg equilibrium. The expected heterozygosities for these loci were 0.9439, 0.9356 and 0.9304; the calculated polymorphism formation content (PIC) was 0.9905; the discrimination power (DP), 0.9998; the paternity exclusion (PE), 0.9572. In addition, significant difference was found in comparison with other populations, and in the sample of families ( n =42) no new mutations could be found. Conclusion The multiplex examination of 3 STR loci can be used in forensic identification and population genetics research.