口腔鳞癌术前放化疗和未治疗患者TIL增殖及特异性杀伤活性的比较

Proliferation and specific cytotoxycity of TIL from OSCC treated with and without radiotherapy or chemotherapy

目的 探讨术前做过放化疗口腔鳞癌患者肿瘤浸润淋巴细胞 (tumorinfiltratinglymphocyte,TIL)在体外能否继续增殖及恢复特异性杀伤活性。方法 从 15例口腔鳞癌原发灶中分离TIL ,比较术前放化疗和未治疗患者TIL体外增殖能力及对自体肿瘤细胞的杀伤活性。结果 术前未治疗组比治疗组提前一周达增殖高峰 ,但 4周以后术前治疗组也显示出继续扩增的趋势。培养 3周时治疗组TIL对自体肿瘤的杀伤活性 <未治疗组(P <0 .0 5 ) ,分别为 2 6 %和 35 %;5周时治疗组TIL对自体肿瘤的杀伤活性与未治疗组无显著性差异 (P >0 .0 5 ) ,分别为 41%和 45 %。结论 从术前放化疗患者肿瘤组织中分离的TIL在体外扩增后转输体内是可行的。

Objective To investigate whether TIL obtained from patients receiving radiotherapy and /or chemotherapy before operation could continue to proliferate and restore specific cytotoxicity.Methods TIL were isolated from fresh tumor tissues in 15 patients with OSCC.The proliferation of TIL from two group patients was compared.The cytotoxicity of TIL from primary OSCC previously treated with radiotherapy or/and chemotherapy and without receiving any treatment was detected using 3 H-TdR 4h-release method.Results TIL from patients previously treated expanded slower and reached proliferation peak one week later than from patients without receiving any treatment,but the former also exhibited continuing expansive trend after four weeks.Culturing for 3 weeks,the cytotoxicity against autologous tumor cells of TIL from patients previously treated was stronger than from patients without receiving any treatment,26% vs 35%(P< 0.05 ),while culturing for 5 weeks,they were 45% and 41% respectively(P> 0.05 ).Conclusion It is feasible to transfer TIL obtained from patients receiving radiotherapy and /or chemotherapy before operation into body.