多塞平对急性脑缺血再灌注大鼠脑组织单胺类递质含量的影响

Effects of doxepin on monoamines transmitter in rat brain after acute cerebral ischemia-reperfusion

目的 :观察多塞平对急性脑缺血再灌注大鼠各脑区单胺类递质含量的影响。方法 :以阻断四血管方法造成急性脑缺血再灌注模型。动物随机分成假手术组 ,空白对照组 ,尼莫地平 3mg·kg- 1组和多塞平 15 ,10 ,5mg·kg- 1组共 6组。在缺血前及再灌注前分别腹腔给药 ,用荧光分光光度计测定大脑皮层、海马、纹状体、脑干中 5 羟色胺、去甲肾上腺素及多巴胺的含量。结果 :与空白对照组比较 ,多塞平 15 ,10 ,5mg·kg- 1剂量组及尼莫地平 3mg·kg- 1组大脑皮层、海马、纹状体各单胺类递质含量显著升高 (P <0 .0 5或P <0 .0 1) ,脑电活动亦显著恢复 (P<0 .0 5或P <0 .0 1)。结论

AIM: To observe the effects of doxepin on monoamines transmitter in the rat brain after acute cerebral ischemia reperfusion. METHODS: Acute cerebral ischemia reperfusion injury was produced by 4 vessels occlusion. Rats were divided into six groups: sham operation group, blank contral group, nimodipine 3 mg·kg -1 group and doxepin 15, 10, 5 mg·kg -1 groups. Drugs were administered ip to the rats before ischemia and before reperfusion. The contents of monoamines (5 HT, DA, NE) in cerebral cortex, hippocampus, striatum and brain stem were determined by fluorospectrophotometry. RESULTS: Compared with blank control group, the contents of monoamines transmitter in cerebral contex, hippocampus and striatum tissues of doxepin 15, 10, 5 mg·kg -1 groups and nimodipine 3 mg·kg -1 group increased significantly ( P <0.05 or P <0.01). Activity of EEG was recovered significantly ( P <0.05 or P <0.01). CONCLUSION: The protective effects of doxepin on acute brain ischemia reperfusion injury are related to its effects on monoamines transmitter.