摘要
采用高效液相色谱法测定骨骼肌腺嘌呤核苷酸和辅酶Ⅰ含量。高压液相系 统包括:惠普公司ZOBAX-C18反相柱5mm×12cm,590UV检测器。流动相为220mmol/L磷酸钾缓冲液,内含10%甲醇,用四丁基氢氧化胺TBAH调pH至65。等比洗脱,流速为08ml/min。检测波长为254nm。所有样品和标准品在进样前均用045μm孔径的虑膜过滤后进样5μl作色谱分析。结果表明:HPLC可以同时测定ATP、ADP、AMP、NAD+、NADH,分离效率高,操作简便,快速每个样品仅需15分钟,而且,重现性好,定性可靠,定量准确。
ATP, ADP, AMP, NAD^+,NADH in skeletal muscle were assessed by an isocratic HPLC separation with UV detection. The HPLC system consisted of a ZOBAXò■-C18 reversed- phase column(Smmxl2cm) and a 590 UV detector. The separation was carried out with 22Ommol/ L sodium phosphate buffer containing 10% methanol adjusted by TBAH to pH6.5. Flow rate was 0.8m1/min. Indirect CV detection was operated at 254nm and reference wavelength at 500, 1OOnm for all experiments. The injection volume was 5μl. This method proved to be reliable and reproducible.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2002年第1期57-60,共4页
Chinese Journal of Sports Medicine