氯对甲型肝炎病毒抗原性及核酸多态性影响的研究

Effects of chlorine on the antigenicity and RNA polymorphism of hepatitis A virus

为了探讨氯灭活甲型肝炎病毒 (HAV)的机制 ,采用酶联免疫吸附试验和RNA的随机引物扩增指纹图谱技术分别检测在氯灭活HAV前后病毒抗原以及核酸多态性的变化。结果显示 :灭活病毒的感染性(加氯 10mg L或 2 0mg L接触 30min)先于破坏病毒的抗原性 (加氯 10mg L或 2 0mg L接触 6 0min) ,当病毒感染性被灭活时 ,往往可以看到病毒核酸多态性的变化。结果表明 :氯灭活HAV可能是通过破坏核酸局部片段所致。

The mechanisms of HAV inactivated by chlorine was investigated by enzyme-linked immunosorbent assay and RNA fingerprinting. Arbitrary primed PCR were involved to detect the changes of antigenicity and RNA polymorphism respectively before and after inactivation by chlorine. The results showed that the infectivity was completely inactivated at 10 mg/L or 20 mg/L of chlorine for 30 minutes and the contacting time should be prolonged to 60 minutes when the loss of antigenicity was observed at the same condition. The changes of RNA polymorphism were often observed while the infectivity was completely inactivated except the concentration of chlorine was 10mg/L for 30 minutes. The results implied that HAV inactivation by chlorine might be the result of destruction of some fractions of HAV genome but not the whole HAV genome.