不同肠段对槲皮素、芦丁吸收的比较研究

Study on the absorption of quercetin and rutin at different segments of intestine

为了比较不同肠段对槲皮素及芦丁的吸收情况 ,选用Wistar大鼠 72只 ,随机分为对照组、槲皮素组及芦丁组 ,每组分别进行十二指肠、空肠、回肠及结肠灌流。灌流液分别为 1%的二甲基亚砜溶液 (溶剂 )、2 0 μmol L槲皮素、芦丁溶液。灌流结束后 ,采用高效液相色谱仪分析灌流液中槲皮素或芦丁的含量。结果发现 :十二指肠、空肠、回肠、结肠对槲皮素及芦丁均有吸收 ,芦丁的吸收率显著低于槲皮素。槲皮素单位长度吸收率 :结肠 >回肠 >十二指肠 >空肠 ,单位面积吸收率 :回肠 >空肠 >十二指肠 >结肠。槲皮素肠道灌流后 ,高效液相色谱图中出现新的未知峰 ,推测其为肠粘膜代谢后形成的衍生物重新分泌进入肠腔所致。

The absorption of quercetin and rutin at different segments of intestine was examined. Seventy two Wistar rats were divided randomly into 3 groups (control group, quercetin group and rutin group), each group was divided into 4 subgroups (duodenum group, jejunum group, ileum group and colon group). Different solutions (1% dimethyl sulfoxide, 20 μmol/L of quercetin and rutin) were perfused respectively. At the end of perfusion, effluent solution samples were analyzed with HPLC. The results showed that both quercetin and rutin could be absorbed at different segments of intestine, but the absorption of rutin was significantly lower than quercetin. On the length basis, the absorption rate of quercetin was colon>ileum>duodenum>jejunum. On the area basis, the absorption rate was ileum>jejunum>duodenum> colon. After the perfusion of quercetin, there were unidentified peaks appearing on HPLC chromatogram. The results suggested that quercetin was derived from intestine and then re\|excreted into intestinal lumen.