微量法检测指血氟和血清氟的分析评价

Evaluation and analysis on the micro-determination of fluoride in serum and finger blood

为探讨指血氟和血清氟的关系 ,选不同接触氟的人群 77名和非接触氟者 13名 (D组 ) ;接触氟的人群分为直接接触氟 (A组 )、间接接触氟 (B组 )及其他工人 (C组 )三组。采集手指血和静脉血并分离出血清 ,用微量载样平口套头盐桥而形成微量加标对电极法 ,测定各组血清氟和指血氟。结果显示 ,指血氟含量在存放前后有明显差别 ;存放前当日测定的指血氟 ,在对照组与各氟接触组之间具有明显差别 ,依次为A >B>C >D组。血清氟比当日指血氟低。血清氟与当日指血氟的相关性较好 (r=0 88) ,回归方程为Y =0 6 2X+0 0 2 3。结果提示 ,当日检测的指血氟可代替血清氟来反映氟接触程度及机体氟负荷水平。

The relationship of fluoride concentration between blood serum from vein and blood from finger tip was explored. Ninety people exposed to different levels of fluoride were selected. Group A was exposed directly to high level of fluoride, group B was exposed indirectly to median level of fluoride, group C was exposed to low level of fluoride and group D had no fluoride exposure. The fluoride concentration was determined by using a modified micro-fluoride electrode. The concentration of fluoride changed obviously with the delay of reservation. The fluoride concentrations of finger blood tested within one day from the four groups were different (A>B>C>D). The fluoride concentration in serum was lower compared with that in finger blood. The results showed that serum fluoride was correlated with finger blood fluoride within one day of reservation ( r =0 87). The regression equation was Y=0 62X+0 023 . It was concluded that the determination of fluoride from finger blood could substitute for serum to reflect the level and degree of fluoride burden of body.