白癜风皮损中NPYVIP和SP的研究

Neuropeptide Y, Substance P and Vasoactive Intestinal Polypeptid in Lesion of Vitiligo

目的 探讨NPY(神经肽Y )、VIP(血管活性肠肽 )、SP(神经肽P物质 )在白癜风发病机制中的作用。方法 采用SABC免疫组化法对 2 0例白癜风患者 (活动期 10例 ,稳定期 10例 )的皮损、非皮损区以及 10例正常人皮肤中的NPY、VIP和SP进行研究 ,并测定各组皮肤标本中NPY、VIP及SP的免疫反应性。结果 活动期白癜风皮损中NPY、VIP及SP的免疫反应性明显增强 ,与正常对照及未受累皮肤比较差异有显著性 ,NPY与SP的反应在白癜风活动期皮损与稳定期皮损比较差异也有显著性。VIP的免疫反应性在白癜风活动期皮损与稳定期皮损中相比稍增强 ,但差异无统计学意义。结论 神经多肽与白癜风的发病有关 ,尤其与白癜风的活动性有关。

Objective To study the role of neuropeptids(NPY,VIP,SP) in the pathogenesis in vitiligo. Methods NPY?VIP and SP immunoreactivity were studied in skin biopsies from lesional skin and uninvolved vitiliginous skin in 20 cases with vitiligo,among them,10 were active,10 were static vitiligo,and in 10 normal controls. The immunohistochemistry technique was employed to measure the antibodies against NPY, VIP and SP.Results An increase in reactivity against NPY,SP, antibody was seen in patient with active vitiligo in the lesional skin and VIP,compared with that in control skin and unvolved skin. Conclusion These findings support the concept of neuronal or neuropeptide involvement in vitiligo,especially with active,and suggest that NPY and SP may have a role in the pathogenesis of the disease.