体外培养创面成纤维细胞的生物学特性及其意义

The significance and biological properties of wound fibroblasts in vitro

目的 研究创面成纤维细胞的生物学特性有利于揭示创面愈合过程中瘢痕形成的机理。方法 采用体外成纤维细胞培养技术 ,开展创面成纤维细胞的分离培养 ,建立细胞库 ;细胞的生长动力学和增殖型、合成型、收缩型等表型变化 ;细胞因子对不同时期来源创面成纤维细胞增殖活性、蛋白合成及其收缩功能的影响。结果 体外培养的创面成纤维细胞保持其在体内的生物学特性 ;其增殖、合成、收缩等表型间转化存在着一定的规律性 ;不同时期来源的创面成纤维细胞对细胞因子的反应性不同。结论 体外控制条件下创面成纤维细胞的生物学活动及其影响因素在细胞水平上揭示了瘢痕形成的理论基础与调控机理 ,为指导临床防治烧伤后瘢痕增生或

Objective To investigate the biological and molecular mechanism of postburn scar formation.Methods Monolayer fibroblasts were primarily cultured from deep partial thickness wounds at different time points after burn in human skin.A cell bank of wound fibroblasts was established to study the growth dynamics and phenotypic properties of fibroblasts;the effects of cytokine on proliferation,synthesis of collagen and contractile function of fibroblasts on in vitro models.Results Human wound fibroblasts continued to express their characteristics during several passages in culture and maintain their biological properties in vivo.The response to cytokines on fibroblasts from different time points was significantly different,which was probably related to the phenotypic regulation of fibroblasts.Conclusions Those in vitro findings reveal the biological and molecular mechanism of scar formation.Further studies are helpful to decrease hypertrophic scarring and/or scar contracture during deep partial thickess burn wound healing.