摘要
利用Basta抗性选择技术快速检测Bt转基因水稻及杂交后代群体单株。试验表明 ,阳性单株在室内和田间有良好的抗螟虫性能 ;在杂交后代中抗除草剂bar基因和目的基因cryIA(b)紧密连锁与协同表达 ;外源基因在F2和BC1群体中遵循孟德尔分离规律 ;应用杂交、回交育种方法将抗虫基因转育到推广品种中是可行的。
Resistance to Basta has been used to detect Bt transgenic rice and progenies of Bt transgenic rice crossed to rice varieties cultivated in the Huang Huai area. The results indicated that Basta positive plants were highly resistant to stripe stem borer(Chilo suppressalis) in the laboratory and field. cryIA(b)gene inherited and expressed together with the marker gene bar. Mendelian segregation of the marker gene bar was observed in F 2 and BC 1 progenies. This study implicates that it is possible to transfer cryIA(b)gene into other main cultivated varieties through crosses and back crosses.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第2期142-145,共4页
Scientia Agricultura Sinica
基金
国家转基因植物研究与产业化开发专项计划课题 (J99 B 0 18)
山东省自然科学基金资助课题 (Y98D10 0 5 8)
