单抗捕捉ELISA检测AEV抗体方法的研究

Study on a Capture ELISA with McAb for Detection of Antibodies against AEV

本研究初步建立了检测ＡＥＶ抗体的单抗捕捉ＥＬＩＳＡ 方法。ＡＥＶ腹水单抗（腹水效价为１ ∶１×１０６）最佳稀释度为１∶２００，ＡＥＶ抗原工作浓度为１６．０８ μｇ／ｍＬ，血清稀释度为１∶１ ００， 兔抗鸡酶标抗体工作浓度为１∶５００。经阻断试验、交叉试验、重复试验，以及与进口试剂 盒 进行平行检测４０份血清，总符合率为９２．５％，表明该方法特异性强、重复性好、敏感性高， 是一种有应用前景的方法。

： The procedure of capture ELISA for detection of AEV an tibodies were e stablished with the monoclonal antibodies against AEV, and the best concentratio ns of the monoclonal antibodies, the AEV and HRP-Rabbit antichichen were 1/200 , 16.08 μg/mL, and 1/500 respectively.The good stability of capture ELISA were v er ified by inhibited tests, cross tests , reproducible tests,parallel tests with E LISA kit from America (IDEXX).