期刊文献+

抗人p185单克隆抗体的体外直接抗癌效应 被引量:7

Investigation on the anti-cancer activities of anti-p185 monoclonal antibodies in vitro
下载PDF
导出
摘要 目的:对使用“表面表位包埋法”构建成功的抗人肿瘤抗原p185的单克隆抗体(mAb)的抑癌活性进行研究。方法:采用免疫荧光染色法、MTT法、软琼脂培养法及细胞长期增殖法等,测定3株mAb的对高表达p185的乳腺癌细胞及转染p185基因的成纤维细胞的抑制活性,以及mAb对提高癌细胞的化疗药物敏感性的效应。结果:这3株mAb均可特异性地明显抑制癌变细胞的生长,并不同程度上提高癌细胞对化疗药物的敏感性。结论:这几株mAb具有治疗p185高表达肿瘤的应用前景。 To study the anti-cancer activity of the three anti-oncoprotein p185 mAbs established by surface epitope masking method so as to provide some basic information for clinical tumor targeting therapy.Methods: Immnune flurorence staining, MTT assay, soft-ar-gar culture and long-term cell proliferation assay detected the suppressive activities of the mAbs against the breast cancer cells overexpressing p185 and p185-transfected fibroblast cells, and the inducing effects of the mAbs on tumors sensitivity to chemotherapy.Results:The 3 mAbs specifically suppressed tumor cell proliferation and enhanced the sensitivity of tumors to chemotherapeutics. Conclusion:These mAbs have the potentials to develop into the clinical tumor targeting therapeutic reagents of the tumors overexpressing p185.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2002年第1期33-35,共3页 Chinese Journal of Immunology
基金 安徽省科委基金资助项目(No.99-D050)
关键词 P185 单克隆抗体 乳腺癌 抗癌效应 p185 Monoclonal antibody Breast cancer
  • 相关文献

参考文献9

  • 1[1]Hynes N E,Stem D F.The biology of p185/neu/HER/-2 and its role in cancer[J].Biochemical & Biophysical Acta,1994;1198:165
  • 2[2]Ravdin P M,chamness G C.The c-erbB-2 proto-oncogene as a prognostic and predictive marker in breast cancer:a paradigm for the development of other macromolecular marker-a review[J].Gene,1995;159:19
  • 3[3]Drebin J A,Link V C,Stem D F et al.Down-modulation of an oncogene protein product and reservation of the transformed phenotype by monoclonal antibodies[J].Cell,1985;41:695
  • 4[4]Harwerth I M,Wels W,Schlegel J et al.Monoclonal antibodies directed to the erbB-2 receptor inhibit in vivo tumor cell growth[J].Br J cancer,1993;68:1140
  • 5[5]Basetga J,Tripathy D,Mendelsoln J et al,Phase Ⅱ study of weekly intravenous recombinant humanized anti- p185HER2 monoclonal antibody in patients with HER-2/neu-overexpressing metastatic breast cancer [J].J Clin Oncol,1996;14:737
  • 6王琛,李昀,李平,刘兢,杨正洪,吕源冈,朱德厚,张荣兴,姚阳,吴强,管伟宁,杨枫.表面表位包埋法制备肿瘤表面抗原P185^(neuc-erbB-2)单克隆抗体及其性质研究[J].中国免疫学杂志,2000,16(10):539-541. 被引量:10
  • 7[7]Ed Harlow,David Lane.Antibodies,a laboratory manual[ M].New York:Grune Stration,1988:409-412
  • 8[8]Harmerth I M,Wels W,Marte B M et al.Monoclonal antibodies against the extracellular domain of the erbB-2 receptor function as partial ligand agonists[J].J Bio Chem,1992;267:15160
  • 9[9]Tum M.Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays[J].J Immunol Meths,1983;65:55

二级参考文献1

  • 1Shen R Q,J National Cancer Institute,1994年,86期,91页

共引文献9

同被引文献16

引证文献7

二级引证文献30

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部