恶性肿瘤患者的抗Koc自身抗体

Auto-antibody to Koc in patients with cancers

目的:为了探讨恶性肿瘤患者的抗Koc自身抗体,研究其临床意义。方法:在优化重组Koc表达和提纯条件的基础上,以重组Koc为包被抗原,采用所建立的检测IgM、IgG、IgA类抗Koc自身抗体的ELISA技术。结果:发现抗Koc的阳性率与恶性肿瘤的类型有密切关系,以肝癌、直肠癌和肺癌较高,胃癌居中,而食道癌最低,其中后者与健康对照无明显差异。以IgM、IgG、IgA中任一类Ig抗Koc对阳性为抗Koc阳性标本,肝癌、直肠癌和肺癌的阳性率均分别＞50．0%,胃癌为25．5%,食道癌为15．8%,均明显较单一阳性率为高。结论:提示Ig类型抗体同步检测对于提高阳性率具有重要意义,抗Koc不失为恶性肿瘤诊断的一个良好新指标。

To characterize the auto-antibody against Koc for understanding its clinical significance in patients with can-ceis.Methods: Based on screening conditions on Koc (KH domain containing protein overexpressed in cancer) expression and purification, recombinant Koc was used as coating antigen for establishment of EUSA to detect anti- Koc of classes of IgM, IgG and IgA. Results: The positive rates of anti-Koc were related to types of cancers, showing higher rates in hepatocellular cancer, rectum cancer and lung cancer, middle rates in stomach cancer, and lower rates in esophagus cancer. The last one was not significant, compared with normal control. When positive samples were defined as being positive for anti-Koc in at least one of three classes of Ig, the positive rates of hepatocellular cancer, rectum cancer, lung cancer, stomach cancer, esophagus cancer were 56.7% , 52.8% , 50.0% , 25.5% and 15.8% , respectively. Their positive rates were significantly higher than that of IgM, IgG or IgA anti-Koc in these cancers. Conclusion: Synchrotron detection of IgM, IgG anj IgA is important and anti-Koc is a new valuable marker to diagnosis of tumors.