不同血清型空肠弯曲菌免疫大鼠的血清及单个核细胞转移诱发周围神经病变的比较研究

Experimental peripheral neuropathy induced by serum and peripheral blood mononuclear cells immunized with Campylobacter jejuni in rats

目的 观察不同菌株空肠弯曲菌 (CJ)免疫大鼠的血清及单个核细胞 (PBMC)对周围神经致病力的差异 ,从而进一步明确CJ不同菌株与格林 巴利综合征 (GBS)的关系。方法 分别将CJ Pen19、Pen43两种菌株免疫大鼠的血清及PBMC转移注射到健康大鼠坐骨神经外膜下 ,7d后作神经原纤维分离及半薄切片甲苯氨蓝染色观察神经病变率及病理类型 ,对两组结果进行比较。结果 (1)免疫血清组 :CJ Pen19免疫血清诱发 10 0 %的坐骨神经发生严重病变 ,其原纤维病变率高达 87%(其中轴索变性 75 %,髓鞘脱失 12 %) ;而Pen43免疫血清组仅诱发 5 0 %坐骨神经病变 ,且病变程度较轻 (原纤维病变率为 2 7 1%,包括 2 2 8%轴索变性和 4 3%髓鞘脱失 ) ,病变率明显低于Pen19组 (χ2 =743 8,P <0 0 1) ;(2 )PBMC组 :CJ Pen19和Pen43两组比较 ,在相应抗原刺激下的PBMC诱发的坐骨神经病变率依次为 30 %、2 0 %(χ2 =0 2 7,P >0 0 5 ) ,其轴索变性率为 8 0 %、8 2 %(χ2 =0 0 4,P >0 0 5 ) ,髓鞘脱失率为 9 0 %、11 8%(χ2 =5 84,P <0 0 5 ) ;植物凝集素 (PHA)刺激的PBMC诱发 40 %和 30 %的坐骨神经病变 (χ2 =0 2 2 ,P >0 0 5 ) ,其轴索变性率分别为 2 1 0 %、2 1 3%(χ2 =0 0 2 ,P >0 0 5 ) ;髓鞘脱失率为 38 3%、44 7%(χ2

Objective Resently, it had been found that Guillain-Barre syndrome (GBS) is associated with Compylobacter jejuni (CJ), as anti-CJ antibodies have been found in the serum of these patients. Nevertheless, whether the antibodies are related with the pathologic changes in peripheral nerves need to be elucidated. In the study, to clarify the association of CJ serotypes with GBS and to observe the different effects of humoral and cellular immune on peripheral nerve pathologic changes, the authors transferred the serum and peripheral blood mononuclear cells (PBMC) from CJ-immunized rats into naive rats perineurally and compared the characteristic pathologic changes between different groups. Method Two groups were established including the group of injection with serum and the group with PBMC perineurally. Each group subdivided again into CJ-Pen19 and Pen43 groups, respectively. The sciatic nerves were harvested at the 7th day after the injection and were taken the pathologic examination. Results (1) In the groups of CJ-immunized serum injection: severe pathologic change showed in 100% of sciatic nerves and the incidence of pathologic fibers was up to 87%, which included 75% of axon degeneration and 12% of demyelination in CJ-Pen19-subgroup. While in Pen43-subgroup, only the moderate changes happened in 50% of sciatic nerves and the incidence of pathologic fibers was only 27.1%, which included 22.8% of axon degeneration and 4.3% of demyelination (χ 2=743.8, P<0.01). (2) Comparing the groups of CJ-Pen19 and Pen43 with injection of PBMCs: the PBMC stimulated with associated-CJ antigen resulted in 30% and 20% pathologic changes in sciatic nerves (χ 2=0.27, P>0.05),respectively. The incidences of the axon degeneration were 8.0% and 8.2% (χ 2=0.04, P>0.05) and, the demyelinations were 9.0% and 11.8% (χ 2=5.84, P<0.05); the PBMC stimulated with PHA resulted in 40% and 30% pathologic changes in sciatic nerves (χ 2=0.22, P>0.05), the incidences of axon degeneration were 21.0% and 21.3% (χ 2=0.02, P>0.05) and, the demyelinations were 38.3% and 44.7% (χ 2=6.33, P<0.05), respectively. Conclusion The incidence of pathologic changes in peripheral nerves induced by CJ was related closely with the CJ serotypes. The CJ immuned-serum caused axon degeneration seriously, while the PBMC resulted in both axon degeneration and demyelination of the sciatic nerve at the same time.