抑制Jak3激酶活性诱导NALM-6白血病细胞凋亡

Inhibition of Constitutively Activated Jak3 and Induction of Apoptosis in NALM-6 Cell Line

目的 研究 Jak3的活化与急性淋巴细胞白血病 (AL L)之间的关系。方法 在不同浓度的 Jak3抑制剂 AG490处理 Jak3活化的 AL L 前 B细胞株 NAL M- 6细胞后 ,用流式细胞术及噻唑蓝法分析细胞的凋亡、增殖情况。结果 除 5 μm ol/ L 组外 ,经 10、15、2 0 μm ol/ L AG490处理后 NAL M- 6细胞均有明显的亚 G1 峰 ,与 DMSO对照相比 ,凋亡率均有显著增加 (P<0 .0 5 ) ;噻唑蓝法示除 5 μmol/ L 组外 ,与 DMSO对照相比 ,10、15、2 0 μm ol/ LAG490均能显著抑制 NAL M- 6细胞的增殖 (P<0 .0 5 )。以上作用呈剂量依赖性关系。结论 Jak3抑制剂 AG490能抑制 NAL M- 6细胞的增殖并诱导凋亡 ,提示 Jak3的活化与前 B型 AL L

Objective To investigate the relation of Jak3 constitutive activation and acute lymphoblastic leukemia(ALL). Methods NALM 6 cells were treated with varying concentrations of AG490, a Jak3 inhibitor. Apoptosis and proliferation of NALM 6 cells were tested by flow cytometry (FCM) analysis and MTT assay. Results With the exception of AG490 5μmol/L, the AG490 10, 15, 20μmol/L induced a strong apoptotic response in NALM 6 cells by FCM analysis( P <0.05) and significantly inhibited the proliferation of NALM 6 cells by MTT assay( P <0.05). All of the effects were dose dependent. Conclusion Jak3 inhibitor AG490 can inhibit proliferation and induce apoptosis in NALM 6 cells, and Jak3 activation is associated with pre B ALL.