几种肿瘤转移基因变化与卵巢癌转移的相关性

Alteration of Multiple Tumor Metastatic Genes with Correlation to Metastasis in Ovarian Carcinoma

目的 研究 MTA1、nm2 3H1 及 E- Cadherin(E- cad)的表达及突变与卵巢癌转移的相关性。方法 应用 RT- PCR、RT- PCR- SSCP和免疫组化技术 ,对正常卵巢、卵巢癌及其配对淋巴结组织进行 MTA1、nm2 3H1m RNA表达和突变及 E- cad蛋白表达的检测。结果 有转移的卵巢癌原发灶 MTA1m RNA高表达率为 10 0 % (7/7) ,无转移为 38.5 % (5 / 13) ,P=0 .0 10 3。有癌转移淋巴结高表达率为 87.5 % (6 / 7) ,而无癌转移为 2 3% (3/ 13) ,P=0 .0 118。有转移卵巢癌原发灶 nm2 3H1 m RNA低表达率为 10 0 % (7/ 7) ,无转移为 30 % (4/ 13) ,P=0 .0 0 43。有癌转移淋巴结低表达率为 10 0 % (7/ 7) ,无癌转移为 38.5 % (5 / 13) ,P=0 .0 10 2。无转移卵巢癌原发灶 E- cad蛋白表达阳性率为 46 .2 % (6 / 13) ,而有转移者 7例均无阳性表达 ,表达率为 0 ,P=0 .0 44。三基因表达相关性分析表明 ,MTA1与 nm2 3H1 、C- cad呈负相关 (r=- 0 .90 3、- 0 .80 2 ) ,nm2 3H1 与 E- cad呈正相关 (r=0 .72 4)。SSCP分析未发现 MTA1和 nm2 3H1 基因突变。结论 MTA1及 nm2 3H1 、E- cad的表达与卵巢癌转移呈正、负相关关系 ,起着正、负调控的重要作用。

Objective To investigate the expression and mutation of MTA1? nm23H 1 and E cadherin(E cad) genes in ovarian carcinoma (OC) in relation to lymph node (LN) metastasis. Methods A panel of normal ovarian tissues, primary OC specimens and corresponding LNS was examined for mRNA expression and mutation of MTA1 and nm23H 1 and protin expression of E cad genes by using RT PCR, RT PCR SSCP and immunohistochemistry. Results The frequency of MTA1 over expression was 100%(7/7)in primary OC with metastasis but only 38.5%(5/13) in those without metastasis( P =0.0103). Overexpression of MTA1 was observed in 87.5%(6/7) of LNS with metastasis but in only 23%(3/13) of LNS without metastasis ( P =0.0118). In contrast with MTA1, low expression of nm23H 1 mRNA was seen in 7 of 7 OC with metastasis but only in 4 of 13(30%) of those without metastasis ( P =0.0043). Low nm23H1 expression was also seen in 7 of 7 LNS with metastasis but only in 5 of 13(38.5%) nonmetastatic LNS ( P =0.0102).Meantime, no expression of E cad protein was observed in 7 of 7 OC with metastasis but in 6 of 13(46.2%)of those without metastasis ( P =0.044). In correlation analysis of the three genes, MTA1 reversely correlated with nm23H 1 and E cad respectively ( r = -0 903, -0.803), and positive correlation existed between nm23H 1 and E cad (r=0.724).No mutation of MTA1, nm23H 1 and was found by SSCP analysis. Conclusion The mRNA expression of MTA1, nm23H 1 and E cad is positively and negatively correlated with LN metastasis. The expression abnormalities but not the mutations of the three genes are frequent events related to LN metastasis of ovarian cancer.