二乙基亚硝胺诱发的大鼠肝癌的细胞外基质研究

Studis on Extracellular Matrices in Rat Hepatocarcinogenesis Induced by Diethylnitrosamine

目的 研究二乙基亚硝胺诱发大鼠肝癌模型的肝脏中 型胶原蛋白、纤连蛋白和层粘蛋白在肿瘤发生发展的演变过程中的动态变化。方法 应用免疫组织化学技术和图像分析方法了解上述三种细胞外基质在肝脏组织病变区的变化。结果 在转变灶中未见细胞外基质表达。在部分瘤性结节中可见上述三种细胞外基质在肝窦壁上表达明显增强 ,肝窦壁呈毛细血管化。细胞形态分析显示 ,这类瘤性结节中细胞平均周长和面积都较对照组显著增加 ,核 /浆比例显著减小。在细胞外基质表达增强的瘤性结节中 ,细胞增生活跃。而肝癌中这三种细胞外基质减少或消失。

Objective The evolution processes of type Ⅳ collagen(CN),fibronectin(FN),and laminin(LN) in carcinogenesis were studied by the use of diethylnitrosamine induced rat hepatocarinogensis model so as to clarify the action of chemical carcinogens on the mechanisms of the occurrence and development of hepatocellular carcinoma. Methods Immunohistochemical technigue and image analysis were used to demonstrate the change of the above mentioned three extracelluar matrices (ECMs) in histopathological foci. Results There were no ECMs expressed in the altered foci, whereas in some neoplastic nodules the ECMs were expressed intensively along the capillarized sinusoid. Cellular morphological analysis showed that in these nodules the average perimeter and the area of the cell increased significantly,but nucleus/cytoplasm ratio decreased significantly as compared with the controls. In the nodules with intensive expression of ECMs, cell proliferation was active. but in the hepatocellular carcinoma these three ECMs diminished or vanished. Conclusion The ECMs play an important role in the occurrence and development of hepatocellular carcinoma.