臂旁内侧核区微量注射羟基马桑毒素二小时的神经组织学研究

Nerve Tissue Morphological Study of Tutin Microinjection into Pontine NPBM in Two Hours

目的 观察臂旁内侧核区微量注射羟基马桑毒素后的神经组织有无毁损。方法 在注药后 2小时 ,动物出现了非常明显的呼吸效应且机能状态良好时灌注固定 ,分别取材做光镜 (Nissl法染色 )和透射电镜标本切片 ,观察拍照。结果 在光镜和电镜下观察 ,未见 NPBM区注射羟基马桑毒素后神经组织有明显的毁损表现 ,与对侧等量注射生理盐水比较也未发现明显差异。结论 在本实验条件下 。

Objective To observe whether Tutin microinjection into the pontine NPBM respiratory area of rabbit will cause morphological damage to that area two hours later.Methods At two hours after the microinjection of Tutin into NPBM, the experimental effects on respiration came to be remarkable and the physiological condition was well, the rabbit was subjected to morphological sampling then. The sample was cut into slices for LM (Nissl dyeing) and transmission EM observation and photography.Results Under the LM and EM examination, no remarkable morphological damage done by Tutin microinjection into the pontine NPBM was observed. By comparing the Tutin injected side with the other side of NPBM where equal normal saline microinjection was given, no apparent morphological difference could be found.Conclusion In our experiment condition, there was no morphological damage caused by Tutin microinjection into pontine NPBM respiratory area of the rabbit.