两种种植材料对龈下优势菌生长及生长环境的影响

The Influence of Two Implant Materials on the Growth of Three Subgingival Predominant Bacteria

目的 了解种植体周炎发病中受到关注的几种龈下优势菌的生长及生长环境与两种常用种植材料的关系。方法 采用配伍组设计 ,将两种常用金属种植材料纯钛和钛 - 6铝 - 4钒合金试件分别与三种龈下优势菌血链球菌 (S.s)、具核梭杆菌 (F.n)、牙龈卟啉单胞菌 (P.g)及其混合菌共同厌氧孵育 ,选择 2天、7天和 14天三个培养时间段 ,采用菌落形成单位计数法测定培养液中的细菌生长量 ,同时用精密 p H计测定培养液 p H值。结果 实验组与对照组间的细菌生长量及生长环境 p H值均无统计学差异 (P<0 .0 5 )。结论 本实验程期内 ,纯钛和钛- 6铝 - 4钒两种种植材料既未影响各单一菌及混合菌中各组成菌的生长量 ,也末影响细菌培养环境的 p

Objective Commercially pure titanium and titanium alloy as dental implants have show impressive clinical results.Despite the high success rates, some implants do fail. Compared to those studies on osseointegration of implants, the information pertaining to their failure is little. Further studies on the relationship between implant material and micro organism are needed. The purpose of this investigation is to study the effect of two commonly used implant materials Titanium (TA2) and Ti 6AI 4V alloy (TC4) on the growth behaviour of three subgingival predominant bacteria Streptococcus sanguis(S.s), Porphyromanus gingivalis (P.g), Fusobacterium nucleatum (F.n) and their mixture.Methods Under anaerobic condition, bacterial suspensions of S.s, P.g, F.n and their mixture were incubated together with the two implant materials respectively, setting the same bacterial suspensions as controls. After 2, 7 and 14 days,the bacterial growth amount was assayed by means of clone forming unit (CFU) method. The pH value of the bacterial suspension was determined by pH Meter. Results The results showed that there was no difference in amount of bacterial growth or pH value between TA2 group and TC4 group ( P >0.05). There was also no statistically significant change as to the proportion of individual bacteria in bacterial mixture or the pH value of culture suspension. Conclusion Under the condition of this investigation, the two studied implant materials have no examined influences on the growth of the subgingival bacteria and the pH value of their culture environment.