人血浆高密度脂蛋白对大鼠内毒素血症的治疗作用初探

Therapeutic Effect of High-density Lipoprotein of Human Plasma on Rat Endotoxemia

目的 观察人血浆高密度脂蛋白 (HDL)对大鼠内毒素血症的治疗效果。方法 采用鲎试剂法和放射免疫分析法测定对照组〔仅静脉输注内毒素 (5 0 0 EU/kg)〕和治疗组〔血压下降后给予 HDL(75 mg/kg)治疗〕大鼠血压、存活时间、血浆内毒素水平和肿瘤坏死因子 (TNF)浓度。结果 与对照组比较 ,输注 HDL 后治疗组大鼠血浆 TNF浓度明显降低 (P<0 .0 5 ) ,血压下降程度明显减弱 (P<0 .0 1) ,存活时间明显延长 (P<0 .0 1)。但两组大鼠血浆内毒素水平在各时点均无明显变化 (P>0 .0 5 )。结论 人血浆 HDL 不但可以抑制 TNF的释放 ,而且还能提高机体对抗内毒素损伤的能力 ,对内毒素血症具有较理想的治疗作用。其机理可能与 HDL 能抑制

Objective To investigate the therapeutic effect of high density lipoprotein (HDL) of human plasma on rat endotoxemia. Methods Rats were divided into 2 groups randomly, the treated group received bolus intravenous endotoxin (500 EU/kg), then followed by a bolus HDL (75 mg/kg) intravenously as their blood pressure decreased obviously. The control group just received bolus intravenously endotoxin (500 EU/kg) without HDL. Blood pressure, survival time, the concentration of TNF and the levels of endotoxin in plasma were determined using radioimmunoassay and limulus lysate test. Results The decrease of blood pressure was obviously attenuated ( P <0.01), the survival time was significantly increased ( P <0.01) and the concentration of plasma TNF was decreased ( P <0.05) in the treated group, compared with those in the control group. The levels of endotoxin were of no significant difference between the two groups ( P >0.05). Conclusion HDL of human plasma has obvious therapeutic effect on rat endotoxemia it could enhance the resistance of rats to endotoxemia, and the mechanism therein may be related to the inhibition of the release of TNF.