三种抗血小板药物在体外对血小板活化的抑制作用研究

Study on the action of three kinds of anti-platelet drugs for in vitro inhibiting platelet activation

目的 探讨阿司匹林、噻氯匹啶和西洛他唑三种药物在体外对血小板活化的抑制作用。方法 流式细胞术三色分析三种抗血小板药物在体外对活化血小板膜表面纤维蛋白原受体 (FIB R)和P 选择素 (CD62P)表达的抑制作用。结果 三种药物对ADP活化血小板膜表面FIB R和CD62P表达的抑制作用明显不同 ,西洛他唑能显著抑制FIB R和CD62P的表达 ;噻氯匹啶抑制FIB R表达的作用显著 ,但对CD62P表达的抑制较小 ;阿司匹林对FIB R和CD62P表达的抑制作用较差。结论 与阿司匹林、噻氯匹啶相比 ,西洛他唑是一种较强的抗血小板活化药物 ,既可抑制FIB R的表达而降低血小板的聚集性 ,又可抑制血小板的释放反应而降低其促凝血活性 。

Objective To study the action of aspirin, ticlopidine and cilostazol in inhibiting platelet activation in vitro. Methods Three kinds of anti platelet drugs, which inhibited the expression of fibrinogen receptor (FIB R) and P selectin (CD62P) on activated platelets surface in vitro, were analyzed by tri color flow cytometry. Results Three kinds of anti platelet drugs inhibited the expression of FIB R and CD62P on ADP activating platelet membrane surface differently. Cilostazol could inhibit the expression of FIB R and CD62P of the platelets markedly. Ticlopidine could inhibit the FIB R expression markedly, but CD62P indistinctly. Aspirin could not inhibit the expression of either FIB R and CD62P on ADP activating platelets. Conclusion Cilostazol is a strong anti platelet drug as compared with aspirin and ticlopidine. It could not only inhibit FIB R expression of activated platelets, which can prevent platelet aggregation, but also decrease the release reaction and procoagulative activities of activated platelets.