流式原位杂交法检测白血病和骨髓异常增生综合征患者端粒长度的研究

Telomere dynamics of leukocytes in bone marrow with leukemia and myelodysplastic syndromes by flow cytometry

目的 通过流式原位杂交法 (FLOW FISH)研究白血病和骨髓异常增生综合征 (MDS)患者骨髓中白细胞端粒长度的变化。方法 采用荧光原位杂交法结合流式细胞术检测 4 3例血液疾病患者和 4 8名健康对照者骨髓中白细胞的端粒长度。以流式细胞仪测定的细胞平均荧光强度 ,即Q FISH值来表示端粒长度。结果 健康对照组Q FISH值为 0 0 66± 0 0 15 ,与同年龄健康对照相比较 ,除 3例未发生转型的MDS和 2例缓解期慢性髓细胞性白血病 (CML)患者的端粒长度无明显变化外(Q FISH值分别为 0 0 69± 0 0 0 7和 0 0 60± 0 0 0 8) ,其余 3 8例患者 (包括 2例转型为急性髓细胞性白血病的MDS和 2例急变期CML)骨髓中白血病细胞的端粒长度均明显变短 (P <0 0 5 ) ,Q FISH值为0 0 4 7± 0 0 19。 8例白血病患者在初诊和化疗完全缓解后端粒长度的改变无统计学差异 (P >0 0 5 ) ,但慢性淋巴细胞性白血病 (CLL)治疗后端粒有缩短趋势 (由 0 0 5 2变为 0 0 4 8) ,而急性髓细胞性白血病 (AML)治疗后端粒有变长趋势 (由 0 0 4 3变为 0 0 5 6)。健康对照组端粒长度随年龄增加而变短 (r=0 67) ,而患病组端粒长度与年龄不相关 (r =0 14 )。结论 通过FLOW FISH法检测到大部分白血病和MDS患者骨髓中端粒长度比健康人短 ,其?

Objective To study telomere dynamics in bone marrow from patients with leukemia and myelodysplastic syndromes (MDS) by flow cytometry and FISH (FLOW FISH) Methods By FLOW FISH , we detected telomere lengths of leukocytes in bone marrow from 43 leukemia patients and 48 healthy donors The mean fluorescence intensity of cells (Q FISH) was detected by flow cytometer Results Compared with the age matched normal range (0 066±0 015), 38 of 43 patients had shortened telomere length (0 047±0 019), and the remaining patients (3 MDS and 2 chromic phase CML) had normal telomere lengths (0 069±0 007 vs 0 060±0 008) Although no significant difference in telomere lengths could be demonstrated in 8 leukemia patients at diagnosis and follow up, a trend toward telomere shortening was observed in CLL patients with 0 052 vs 0 048 in which telomere tended to shorten as the disease went on In contrast, longer telomeres were found in AML patients after induction chemotherapy (0 056) compared with those found in diagnostic specimens (0 043) In the healthy donors, the telomere length turned to be shorter with increase of age ( r =0 67), whereas in leukemia and MDS patients, the telomere length had no correlation with age ( r =0 14) Conclusion The telomere lengths from most patients with leukemia and MDS were shorter than that of healthy donors The telomere dynamics is associated with prognosis and therapy of disease