流式细胞技术在移植术后受者巨细胞病毒诊断上的应用

The use of flow cytometry for the detection of CMV in recipients of transplantation

目的 探讨流式细胞技术 (FCM)诊断移植受者术后巨细胞病毒 (cytomegalovirus,CMV)的感染状况。方法 应用酶联免疫吸附方法、免疫组化方法及流式细胞技术测定 79例移植受者术后抗巨细胞病毒抗体 (CMV Ab)及巨细胞病毒抗原 (CMV Ag)。结果 移植受者抗CMVIgG和抗CMVIgM阳性率分别为 10 0 %和 2 5 %免疫组化方法检测CMV抗原阳性细胞数 0～ 2 0个以上 / 5× 10 4WBC ,而随着CMV抗原阳性细胞数的增多 ,FCM测定CMV抗原的阳性率也逐渐升高。结论 移植受者术后存在不同程度的CMV感染 。

Objective Use flow cytometry to detect cytomegalovirus antigen in recipients of transplantation. Methods The CMV Ab and CMV Ag in 79 recipients of transplantation were detected by ELISA、immuno histochemistry and flow cytometry. Results The positive of anti CMV IgG and IgM was 100% and 2.5% respectively; and the number of CMV positive cells detected by immuno histochemistry were 0 to more than 20/5×10 4 WBC. A comparison with the immuno histochemistry assay showed that the more CMV positive cells, the higher positive rate detected by FCM. Conclusion CMV infection were very common in the transplant patients, the advanced technology of FCM carried out to detect CMV specific antigen was more significant to diagnosis CMV infection in recipients of transplantation.