肝活检组织乙肝病毒核心抗原定量检测方法的建立

The Establishment of the Special-Qualitative-Quantitative-Analysis-Method of HBcAg for Liver Biopsy Tissue with CHB

目的 探讨建立对乙肝活检组织乙肝病毒核心抗原 (HBcAg)定性、定量检测的诊断方法。方法 将 3 7例慢乙肝患者肝活检组织制备成单细胞悬液 ,用小鼠抗人HBcAg单克隆抗体标记后 ,应用流式细胞分析术进行了定性定量检测 ,并制定了诊断标准。结果 该方法对肝穿活检组织HBcAg定性检出率与临床常规乙型肝炎五项检验阳性检出的符合率为 10 0 % ,病理学分期Ⅰ、Ⅱ期的慢乙肝肝活检组织HBcAg指数 (HBcⅠ )分别为 :3 .4 9± 1.5 5、9.3 7± 3 .15 ,Ⅲ期的 2例分别为 :3 2 .12和18.4 ,统计学证实肝穿活检组织HBcAg流式定量检测与慢乙肝肝活检组织病理分期显著相关 (P <0 0 5 ) ,且与治疗效果显著相关 (P <0 .0 5 ,P <0 .0 1) ,能够作为一种检测疗效的方法。结论 该方法能够对HBcAg定性兼定量诊断 ,又能直接反映肝组织内乙肝病毒的存在 ,与目前较常规乙肝诊断方法相结合 。

Objective To investigate and establish the special qualitative and quantitative analysis method of HBcAg for liver biopsy tissue with CHB Methods Thirty seven patients with CHB were performed liver biopsy and the tissues were made cellular suspension, then immunologically marked by fluorescent monoantibody of HBcAb, at last qualitatively and quantitatively measured by Flow cytometry (FCM) Result This special qualitative analysis method of HBcAg for liver biopsy tissue with CHB was completely related to that of routine clinical test of HBcAg, the HBcAg Ⅰndex (HBcⅠ) by FCM of pathological Ⅰ, Ⅱ stages were respectively 3 49±1 55、9 37±3 15,two cases of Ⅲ stages were respectively 32 12 and 18 4,and the quantitative analysis was statistically correlated with the pathological staging and curative effect of CHB( P <0 05) Conclusion This method could directly determinate the HBcAg in liver tissue Combined with the routine clinical test of HBcAg, this method has important clinic significance