唾液及菌斑液中蛋白成分与龋易感性的关系

Proteins in saliva and dental plaque fluid in relation to caries susceptibility

目的 探讨纯腮腺液、全唾液及菌斑液中蛋白成分与龋易感性的关系。方法 选 39名无龋者 (cariesfree ,CF)和 2 7例高龋者 (cariessusceptible ,CS) ,用Folin酚法测定纯腮腺液、全唾液及菌斑液的总蛋白含量 ,SDS PAGE和图像分析系统定量分析各蛋白成分。结果 总蛋白含量菌斑液高于唾液近 10倍 ,其中仅CF组全唾液与菌斑液有较大相关性 (r =0 80 4)。 3种液体共有的蛋白为140 0 0、6 6 0 0 0及 76 0 0 0蛋白 ,菌斑液中 140 0 0、15 0 0 0及 380 0 0蛋白和全唾液中 140 0 0蛋白的含量CS组显著低于CF组。结论 无龋组菌斑液蛋白受全唾液影响较大。 3种液体中总蛋白含量均与患龋无关 ,但菌斑液和全唾液中某些蛋白可能有重要的抗龋作用。

Objective To investigate the relationship of the levels of proteins in parotid saliva, whole saliva and dental plaque fluid with caries susceptibility Methods Sixty six of university students were selected as subjects,39 in caries free group(CF, DMFS=0)and 27 in caries susceptible group(CS, DMFS≥8 and DT≥3). Total protein concentration was detected with Lowry method Protein compositions were separated with SDS PAGE and alkaline electrophoresis The gels were analyzed using an image evaluation system Results Total protein level in dental plaque fluid was about 10 fold higher than that in saliva Whole saliva was closely related to dental plaque fluid in terms of proteins only in CF group ( r =0 804), but there was little relation in CS group The proteins that occurred in all three fluids were 14 000, 66 000 and 76 000 proteins The 14 000, 15 000 and 38 000 proteins level in dental plaque fluid and 14 000 protein level in whole saliva were significantly lower in CS group than in CF group Conclusions The proteins of dental plaque fluid are influenced significantly by whole saliva in CF group The results suggest some kinds of proteins in dental plaque fluid and in whole saliva might play important roles against caries