睾丸局部感染时Sertoli细胞IL-1、IL-6 mRNA的表达

Immunoregulative mechanism of the Sertoli cell in testis infection by expression of IL-1 and IL-6 mRNA

目的 研究大鼠Sertoli细胞在抗感染中的免疫调节作用。方法 以溶脲脲原体 (ureaplasmaurealyticum ,UU)和致病性大肠杆菌直接注入大鼠膀胱模拟上行性感染的途径 ,分别在 1、2和 3周处死大鼠 ,从大鼠睾丸组织分离获得高纯度的Sertoli细胞 ,然后抽提总RNA ,用RT PCR方法比较正常组与UU感染组、致病性大肠杆菌组之间IL 1、IL 6mRNA表达的差异。结果 与正常组相比 ,UU感染后 ,其IL 1mRNA在 1、2周时升高 ,3周时下降 ;IL 6在 1、2周时下降 ,3周时升高 ;而致病性大肠杆菌感染后 ,其IL 1mRNA在 1、2周时均升高 ,3周下降 ;IL 6在 1周时升高 ,2周时下降 ,3周时又升高。结论 大鼠Sertoli细胞在抗感染免疫中 ,可通过IL 1、IL

Objective:To study the effect of isoflavone and genistein on activation of T lymphocytes in order to develope new immuno intervention reagent.Methods:Fluorescence conjugated monoclonal antibodies and flow cytometer were used to detect the expression rate of CD69 by activated T cells in vitro in response to Phytohemagglutinin(PHA) and Phorbol 12,13 dibutyrate(PDB),with some samples pre incubated with 10,50 or 100 μmol/L of genistein,after 2 h and 6 h of incubation in whole blood culture system.Results:After 2 h of culture,the inhibitory effect in PHA group was stronger than PDB group(P<0.05);after 6 h of culture,the inhibitory effect in PHA group was still stronger than PDB group(P<0.05),but both groups presented decreased inhibition rates compared to 2 h.Conclusion:Genistein inhibited expression of CD69 by activated T cells in response to PHA and PDB in a concentration dependent manner and could be a developed into immuno intervention reagent.