山东省健康儿童肠道病毒带毒状况调查

An Epidemiological Study on Enteroviruses-carrying Status among Healthy Children in Shandong Province

[目的 ]了解山东省健康儿童肠道病毒带毒状况。 [方法 ]按照随机抽样的原则 ,1998年分别于肠道病毒感染的高发季节 ( 7月 )和低发季节 ( 11月 ) ,在 5个县 (市 )随机采集城镇和乡村共 10 16份 15岁以下健康儿童的粪便标本 ,采用Hep 2和RD细胞同时进行肠道病毒的分离、鉴定。 [结果 ]高发季节肠道病毒的带毒率 ( 13 0 8% )高于低发季节( 3 83% ) ,高发季节不同县 (市 )、不同年龄组之间的带毒率不同 (P <0 0 5 ) ,低发季节的差异则无统计学意义 (P >0 0 5 ) ;城镇、乡村带毒率相近 ,但不同县 (市 )城、乡之间仍存在差异 ;男、女之间的带毒率差异无统计学意义 (P >0 0 5 ) ;两次采样分离的肠道病毒均以ECHO病毒为主 ,其次为其他未定型肠道病毒、Coxackie病毒、脊髓灰质炎病毒 (类疫苗株 )和肠道病毒 70型 ;有 9名儿童两次采样均分离到肠道病毒 ,其中 6人两次分离的病毒一致 ,分别为ECHO6、ECHO7、E CHO19、ECHO2 0 和Coxackie病毒B5、B6;两次采样RD细胞的分离率 ( 6 89% )高于Hep 2细胞 ( 3 74% )。 [结论 ]健康儿童肠道病毒带毒 (特别是在夏秋季节 )较为普遍 ,并可能存在长期带毒状况 ;未发现脊髓灰质炎野病毒。

Objective Objective To explore the enterovirus-carrying status among healthy children under 15 year-old in Shandong province Objective [MethodsObjective ]Total 1 016 specimens were collected randomly from 5 counties in high infection season (July) and low infection season (November) of 1998 Hep-2 and RD cells were used together for enteroviruses isolation and identification Objective [ResultsObjective ]The enteroriruses-carrying rate in July (13 08%) was significantly higher than that in November (3 83%) There were significant differences of the enteroriruses-carrying rates for specimens collected in July among different counties and age groups(P<0 05), but no differences in November(P>0 05) The total enteroriruses-carrying rates between towns and villages were almost similar, but there were differences among different counties No significant differences were found between males and females(P>0 05) Echoviruses were the main types among 87 enterovirus isolates Coxackieviruses, polioviruses (vaccine derived strain), enterovirus type 70 and non-identified viruses were other types There were 9 children that enteroviruses were isolated from specimens of the same person collected in July and November Among them, 6 children were isolated the same enteroviruses type, i e , ECHO_6?ECHO_7?ECHO_ 19 ?ECHO_ 20 ?Coxackievirues B_5 and Coxackievirues B_6 respectively The enteroviruses-isolating rate by RD cell (6 89%) was significantly higher than that by Hep-2 cell (3 74%) among total specimens Objective [ConclusionsObjective ]Carrying enteroviruses was not only common among healthy children especially in high infection season, but also maybe exist the long-term carrying status No wild polioviruses were found