DNA损伤修复能力对GPA基因突变频率的影响及其检测方法

The influence of DNA repair ability on GPA mutation frequency and it's detection

血型糖蛋白 A(GPA)基因突变频率在作为生物剂量计及癌的风险预测方面最大的缺陷在于个体差异大。DNA损伤及修复能力是影响 GPA基因突变频率个体差异的的决定因素之一。随着现代分子生物学实验技术的发展 ,检测 DNA损伤及修复能力的方法不断出现 ,例如中性膜洗脱法、单细胞凝胶电泳、梯度电压凝胶电泳等 ,使得应用 DNA损伤及修复能力对

The prominent disadvantage of using GPA mutation frequency as biodosimeter and cancer risk predictor is the significant individual differences. DNA damage and repair ability is one of determinants influencing the individual differences in GPA mutation frequency. With the improvement of current protocols for molecular biology, the measurement of detecting DNA damage and repair ability has been made, such as neutral filter elute, single cell gelelectrophoresis, graded voltage gel electrophoresis, which make it feasible to modify the individual difference in GPA mutation frequency with DNA damage and repair.