P物质在单眼剥夺性弱视猫外侧膝状体中的表达

Expressions of substance P in lateral geniculate nucleus in monocular deprivation cats

目的 检测 SP在单眼剥夺弱视猫 L GN中的表达 ,并探讨其在弱视发病中的意义。方法 采用免疫组化 APA法 ,对 7例正常猫和 8例单眼剥夺弱视猫 L GN SP免疫阳性神经元及神经纤维进行定位并定量研究其变化。结果 在单眼剥夺猫剥夺侧 L GN的A1层 SP表达显著下降 (P <0 .0 5 )。结论 单眼剥夺可造成剥夺侧 L GN剥夺眼相应输入层次的 SP表达下降 。

Objective To investigate the changes of substance P (SP) in lateral geniculate nucleus (LGN) in monocular deprivation cats, and discuss its role in the development of deprivation amblyopia. Methods Seven normal cats and eight monocular deprivation cats were processed by immunocytochemical method APA to demenstrate the localization of SP in LGN, and the data were analysed. Results In the monocular deprivation cats, the number and grey level of SP immunopositivity reduced greatly in ispilateral lamina A1 (P<0.05). Conclusion Monocular deprivation in critical period of a newborn may lead to great changes of SP in LGN A1,which receives imputs from the deprived eye. So it can play a role in the development of amblyopia.