过敏性哮喘患者血清TIgE、IgG_4、ECP水平变化及意义

Change and significance of TIgE, IgE, IgG_4 ECP in serum of allergic asthmatics

目的:通过观察能反映细胞免疫和体液免疫状态的三项指标(TIgE、IgG_4和ECP)在过敏性哮喘发作时血清水平的变化,进一步探讨过敏性哮喘的免疫学发病机制。对象和方法:分别选择过敏性哮喘轻,中度发作期和缓解期患者各30例,并选15例健康体检者做对照,入选者采用ELISA法测定血清TIgE、IgG_4水平。采用Immuno—CAP检测系统测定血清ECP水平。结果:过敏性哮喘发作期及缓解期血清TIgE与IgG_4水平均升高,且趋势一致,与正常对照组比较,差异显著(P<0.01-0.05)。发作期ECP明显升高,与缓解期及对照组相比均有显著性差异(P<0.01-0.05)。发作期中TIgE与IgG_4间存在正相关关系。结论:本研究选择观测指标(TIgE、IgG_4和ECP)能较全面地反映过敏性哮喘患者体内EC、MC和T—LC的免疫功能状态,并从不同侧面反映过敏性哮喘发病过程细胞和体液免疫功能的变化,从实验结果可以看出,免疫学发病机制在过敏性哮喘发病中的地位和作用。

Objective To observe the serum level of some indexes which maybe reflect the state of cellular and humoral immune in asthmatics and study further on the immunologic pathogenesis of allergic asthma. Method Each 30 cases of allergic asthmatics with a-cute attacks from mild to moderate degree(group A)and with remission(Group B)Were chosen respectively, while choice of 15 healthy controls (group C).The total immunoglobulin E(T IgE),immuno-globulin G4(IgG4)and eosinophil cationic protein(ECP)levels in the serum were measured with either ELISA or Immuno-CAP System. Result of serum T IgE and IgG4 levels in group A and B were significantly higher than in group C. There were significant difference between A and C,B and C(P<0.05 - 0.01). Conclusion The indexes in this research have reflected comprehensively the immune functional state of EC, MC and T-LC, and reflected the change of cellular and humoral immune function in allergic asthmatics from different sides. Our result shows that the immunologic mechanism play an very important role in the pathogenesis of allergic asthma.