缺血-再灌注肝脏组织中ICAM-1基因的表达

Expression of ICAM-1 gene in the ischemia - reperfusion liver

目的 :探讨肝脏缺血 -再灌注过程中 ,肝窦内皮细胞ICAM - 1mRNA的表达规律及其意义。方法 :应用分子杂交技术 ,观察缺血时间分别为 15、3 0min及 4 5min的 3组兔肝脏于再灌注 60min时ICAM - 1mRNA的表达情况 ,并对肝窦腔内的白细胞数量进行计数。结果 :3组肝脏缺血前及缺血末组织内仅有少量ICAM - 1mRNA表达于肝窦内皮细胞浆内 ,且肝窦腔内的白细胞数量也无明显改变 ;但于再灌注 60min时 ,ICAM - 1mRNA表达程度则显著增强 ,且缺血时间越长的肝脏 ,其表达强度越大。此外 ,组织内ICAM - 1mRNA含量越高的肝脏 ,肝窦腔内白细胞数量越多 ,两者呈显著的正相关关系。结论 :肝脏的缺血能明显诱导再灌注期间肝窦内皮细胞表达ICAM - 1,增强肝窦内皮细胞的粘附力 ,促进再灌注血流中的白细胞在肝窦内滞留 ,进而引发一系列病理生理改变。

AIM: To investigate the expression of ICAM-1 mRNA in hepatic sinusoidal cells (HSCs) during liver ischemia-reperfusion injury and its effects. METHODS: Three groups of rabbit livers experiencing ischemia for 15, 30 and 60 minutes respectively, and reperfusion for 60 minutes were conducted. The hepatic ICAM-1 mRNA expression was observed and leukocytes in hepatic sinus were counted. RESULTS: The lower expression of ICAM-1 mRNA showed in HSCs before reperfusion. Besides, leukocyte counts could no change. However, the more expression of ICAM-1 mRNA was found at the point of 60 minutes reperfusion. Furthermore, the higher expression of ICAM-1 mRNA showed in the more prolonged ischemic liver. Meanwhile, there was significant positive correlation between ICAM-1 mRNA contents and leukocyte counts. CONCLUSION: The high ICAM-1 mRNA expression induced by hepatic ischemia in HSCs increased adherence of HSCs and sequestrated more leukocytes in liver.