高速逆流色谱溶剂体系软件在桂枝汤A部分研究中的应用

Application of the Solvent Systems Software of High-speed Countercurrent Chromatography in Studying the Active Fraction A of Guizhi Decoction

目的 :介绍一种快速选择和评价高速逆流色谱溶剂体系的方法。方法 :利用化工热力学溶液理论 ,结合溶剂体系模型函数 ,设计了溶液体系软件 ,计算出溶剂体系上下相组成和关键参数 (如体积、粘度、介电常数、极性参数等 ) ,定量评价了溶剂体系。结果 :以中药复方“桂枝汤”A部分为研究对象 ,论证了该软件应用于选择和优化HSCCC溶剂体系分离中药复方的适用性。结论 :该软件对选择和优化高速逆流色谱溶液体系具有很好的指导作用 。

Objective: To introduce a new way, which facilitates the systematic search and evaluation of the solvent systems suitable for high-speed countercurrent chromatography (HSCCC). Methods: The software integrated with the thermodynamics solution functions and theory is directive to calculate the composition, physical parameters, such as volume, viscosity, dielectric constants, and polarity parameters of the upper and lower phases. Results: Separation of Guizhi-decoction Fr. A by HSCCC, one of traditional Chinese medcines, is conducted to prove the applicability of this software in selection and optimization of solvent systems. Conclusion: This software can be used to select and optimize the solvent systems of HSCCC. It is supposed to extend this method.