黄芪总黄酮对大鼠佐剂性关节炎的治疗作用及机理研究

Studies on the Therapeutic Effects of Total Flavonids of Astragalus on Adjuvant Arthritic Rats and Its Mechanism

目的 :研究黄芪总黄酮 (TFA)对大鼠佐剂性关节炎 (AA)的治疗作用及机理。方法 :检测大鼠非致炎侧足爪体积 ,血清丙二醛 (MDA) ,滑膜细胞产生白细胞介素 1(IL 1)和亚硝酸盐 (NO-2 )量。结果 :AA鼠血清LPO、滑膜细胞产生IL 1和NO-2 量明显升高 ,且MDA和IL 1与AA鼠非致炎侧足肿胀度呈明显正相关 ,TFA全程或一周的阶段性治疗在发挥抗炎作用的同时 ,均可使AA鼠过高的MDA ,IL 1和NO降低。结论 :TFA对大鼠佐剂性关节炎有治疗作用 ,此作用可能与其降低血清LPO ,滑膜细胞产生的IL 1和NO量有关。

Objective: To study the effect of total flavonoids of astragalus (TFA) on adjuvant arthritic (AA) rats and its mechanism. Methods: The volume of non-injected hind paw of AA rats, serum malondialdehyde (MDA) content, interleukin-1(IL-1) and nitrite (NO - 2) produced from articular synoviocytes were measured. Results: It was obseved that serum levels of MDA and the levels of IL-1 and NO - 2 from synoviocytes increased in AA rats, and the degree of the secondary inflammatory reaction of AA rats appeared to be directly correlated with serum levels of MDA and IL-1. Treatment of whole (d0～27) or partial (d12-18 or d18-24) course of AA rats with TFA (20 mg/kg/d,ig) could not only markedly inhibit the inflammatory reaction in AA rats, but also reduce their enhanced serum lipid peroxides (LPO), IL-1 and NO production from synoviocytes. Conclusion: TFA has significant therapeutic effects on AA rats, which might be related to both of anti-oxidative effect and the reduced production of IL-1 and NO from synoviocytes.