一步法RT-PCR检测石蜡包埋滑膜肉瘤中SYT-SSX融合基因的表达

Detection of SYT-SSX Fusion Transcript in Synovial Sarcoma by One-Step Reverse Transcriptase-Polymerase Chain Reaction

运用一步法RT PCR技术检测 14例石蜡包埋滑膜肉瘤组织中t(x ;18) (p11.2 ;q11.2 )染色体易位所致的融合基因SYT SSXmRNA的表达 ,尤文氏肉瘤和恶性黑色素瘤各一例做对照。结果表明 ,14例滑膜肉瘤中 12例有SYT SSX融合基因的表达 (85 7% ) 。

A characteristic SYT SSX fusion transcript resulting from the chromosomal translocation t(x;18)(p11.2;q 11.2 ) is detected in fourteen cases paraffin embedded synovial sarcoma by one step reverse transcriptase polymerasechain reaction . One case of Ewsing'sarcoma and melanoma was detected as control. Results shows that SYT SSX fusion transcript was expressed in twelve of fourteen cases (85.7%),but did not find in Esing's sarcoma and melanoma.