大鼠局灶性脑缺血早期脑内HO-1蛋白的表达

Neuronal Expression of Heme Oxygenase Protein During Early Focal Cerebral Ischemia in Rats

目的 研究局灶性脑缺血血红素氧合酶 (HO)在脑内的表达。方法 采用大鼠大脑中动脉栓塞致脑缺血 ,对缺血早期不同时间点进行HO 1免疫组化及病理学研究 ,并应用计算机图像分析技术计算HO 1表达的强弱。结果 栓塞后 3 0min皮质及海马即有HO 1阳性神经元和胶质细胞 ,且随着时间推移 ,HO 1的表达逐渐增强 ,HO 1主要分布在灶周皮质、梗死皮质区、梨状皮质内的神经元及胶质细胞 ,丘脑、下丘脑、海马齿状回的神经元及对测海马也出现HO 1的表达。结论 脑缺血时脑内HO

Purpose: To study the expression of heme oxygenase protein during early focal ischemia in rats. Methods: The expression of heme oxygenase(HO)-1 protein during early focal cerebral ischemia was investigated by using immunohistochemistry and computer image analysis in middle cerebral artery occlusion in rats. Results: HO-1 expression was observed in the neurons and gliacytes of the ipsilateral cerebral cortex infarction area and hippocampus at 30 miniute after occlusion. With time going on, the expression of HO-1 increased, HO-1 expression was observed in the ipsilateral infarction border area, frontal and cingulated cortex, thalamus, hypothalamus and bilateral hippocampus. Conclusions: Strong and long-lasting expression of HO-1 protein is evidently significant physiological consequences in the recovery of neuronal tissue from ischemic injury in focal cerebral ischemia.