乙型肝炎病毒序列准种个体化特征的研究

THE PRELIMINARY STUDY ON INDIVIDUALLY CHARACTERIZED QUASISPECIES OF HEPATITIS B VIRUS

以乙型肝炎病毒(HBV)多聚酶 (P)逆转录酶 (RT)区及表面抗原 (HBsAg)主蛋白、e抗原 (HBeAg)氨基酸序列异质性来探讨HBV准种群在感染个体中的变异特点。应用多聚酶链反应 (PCR)方法自 8例慢性HBV患者血清中扩增靶基因 ,克隆入T载体 ,随机挑选 2 7株克隆测序 ,将获得基因的推断氨基酸序列进行比较后发现 :病毒结构/非结构蛋白氨基酸序列存在广泛的变异现象 ,替换突变表现出一定的个体特异性 ,其结果是导致HBV在特定的患者体内可能存在特征性的变异。本研究提示HBV在患者体内的蛋白序列多样性是乙型肝炎慢性化的一个重要原因。

Primers were synthesized according to DNA sequence of HBV found in China, and the reverse transcriptase (RT) region in polymerase gene, HBV preC/C region and the whole genome were amplified by PCR method from the serum of 8 patients with chronic HBV infection. Then the PCR products were ligated into pGEM Teasy vectors. 27 clones were sequenced. Deduced amino acid sequences were compared, and the results showed that mutations were widely distributed in structural and non structural viral proteins. The substitution mutations occur in clones isolated from specific patients, which led to a characteristic mutation in the quasispecies group. The results indicated that the diversity of HBV gene might play an important role in the pathogenesis of chronic HBV infection.