人类胚胎低温保存溶液EFSh40实现玻璃化工况条件的实验研究

STUDY ON WORKING CONDITIONS UNDER WHICH SOLUTION EFSh40 VITRIFIES

实验研究了溶液EFSh40实现玻璃化的工况条件以及人类胚胎低温保存中采用双层麦管的可行性。研究结果表明 ,玻璃化溶液EFSh40在降温速率高于1 0 0K/min时能够玻璃化 ,复温速率高于 2 0 0K/min时没有反玻璃化现象。本研究也实现了双层麦管中溶液EFSh40的玻璃化。采用双层麦管不但避免了潜在的污染问题 。

Experiments were conducted to study working conditions under which solution EFSh40 vitrifies EFSh40 is a vitrification solution developed to cryopreserve human embryo The feasibility of applying double straw was studied as well EFSh40 will vitrify above the cooling rates of 100 K/min and will not devitrify above the warming rates of 200 K/min t Meanwhile EFSh40 also vitrifies in double straw Double straw will not only avoid potential contaminations,but also simplifies the operation processes