模拟高原训练对大鼠促红细胞生成素(EPO)表达的影响

Effects of Mimic Altitude Training on the Expression of Rat Erythropoietin (EPO)

为了进一步研究高原训练对大鼠红细胞生成的影响，本研究以大鼠１８Ｓ－ｒＲＮＡ为内参照，用定量反转录聚合酶链式反应（ＱＲＴ－ＰＣＲ），测定了６０只ＳＤ雄性大鼠在模拟不同海拔游泳训练后ＥＰＯ ｍＲＮＡ的变化。结果表明，所有高原对照组中ＥＰＯ ｍＲＮＡ水平均高于平原对照组，其中高原３０００米组和４０００米组较平原对照组有显著性增加Ｐ＜０．０５。在高原训练组中，除了高原２０００米训练后１周组及２周组较高原２０００米对照组ＥＰＯ ｍＲＮＡ水平有较大幅度增加外，其余各高原训练组ＥＰＯ ｍＲＮＡ水平较该组对照均有不同程度的下降。其中高原３０００米训练后１周组及２周组较高原３０００米对照组ＥＰＯ ｍＲＮＡ水平有显著水平的下降Ｐ＜０．０５，而高原４０００米训练后１周组及２周组较高原４０００米对照组ＥＰＯ ｍＲＮＡ水平有非显著水平的下降Ｐ＞０．０５。

In order to further characterize the influence of altitude training on the proliferation and differentiation of erythroid progenitor cells, various EPO mRNA levels of rats trained at different altitudes were detected by the quantitative reverse-transcription polymerase chain reaction(QRT-PCR)with the 18S-rRNA gene of rat as reference. Results showed that the EPO mRNA output of all altitude control was higher than that of plain control,and the EPO mRNA production of altitude controls at 3km and 4km increased significantly compared with non-altitude control(P<0.05). EPO expression decreased one or two week after altitude training at 3km and 4km in comparison to the corresponding altitude controls, while increased greatly at 2km and the reduction in 3km group was significant(P<0.05).