致病性弧菌的分离鉴定及耐药性分析

Isolation and identification of pathogenic vibrios and analysis for drug tolerance

目的 了解夏秋季引起腹泻的病人中致病性弧菌的分布及其耐药性。方法 应用 6种培养基和 19种生化鉴定管分离培养致病性弧菌 ,并对所鉴定的细菌用K B法作了 14种抗生素的体外药敏实验。结果 共检出 187株致病性弧菌 ,总分离率为 19.4%。其中检出弧菌属细菌 138株 ,以副溶血性弧菌为主 ;气单胞菌属 44株 ,以温和气单胞菌为主 ;类志贺邻单胞菌 5株。药敏结果表明 ,青霉素类、头孢一代磺胺类等抗生素耐药率较高 ;喹诺酮类、头孢三代、氨曲南以及亚胺配能等抗生素抗菌活性较强。结论 应用多种培养基和生化鉴定管分离培养致病性弧菌可检出大多数弧菌科细菌。对由致病性弧菌引起的腹泻应首选环丙沙星和亚胺配能 ,氨曲南和头孢他啶可作为二线药物应用 。

Objective To investigate the distribution and drug tolerance of pathogenic vibrios in patients with diarrhea in summer and autumn seasons.Methods Pathogenic vibrios were isolated by six kinds of culture mediums and nineteen kinds of biochemical idendification tubes,and the vibrios isolated were tested for sensitivity to fourteen kinds of antibiotics by K B method.Results 187 strains of pathogenic vibrios were isolated out,general isolation rate was 19.4%,138 strains of vibrio bacteria of which were main parahemolytieus vibrios,44 strains of aeromonas of which were main mild aeromonas and 5 strains of which were shigella aeromonas.The result of drug sensitive test showed that the vibrio's drug fast rates to penicillin drugs,cephalosporin Ⅰ and sulfa drugs were higher;the antibacterial activities of quinotone,cephalosporin Ⅲ,aztreonam and imipenem were more powerful.Conclusion Most of vibriobacteria could be isolated by cultured with various culture mediums and various biochemical idendification tubes.The cinrofloxacin and imipenem should be chosen firstly in treating diarrhea caused by pathogenic vibrios and aztreonam and ceftazidime could be chosen secondly,but ampicillin and cefazolin should not be chosen because the drug fast rates of which were higher.