一氧化氮对内毒素诱导暴发型肝衰竭肝损伤的影响

The Effect of Nitric Oxide on Liver Injury in Fulminant Hepatic Failure Induced by Endotoxin

目的 :检测肝组织一氧化氮合成酶 (nitricoxidesynthase ,NOS)的分布表达状态和肝组织病变情况 ,并检测肝功能 ,研究一氧化氮 (nitricoxide ,NO)对暴发型肝衰竭大鼠肝损伤的影响。方法 :制备亚急性暴发型肝衰竭的动物模型 ,以NOS单抗 ,用免疫组化技术检测。结果 :给药后及肝大部分切除术后不同时期 ,诱导型NOS的变化基本与谷丙转氨酶及总胆红素的变化相平行 ,且与肝组织病变程度相一致。结论

Objective: Our aims were to determine nitric oxide synthase(NOS)expression in surface of hepatocyte and liver histochemical change and to test liver function so as to analyse the role of nitric oxide (NO) in liver injury of fulminant hepatic failure(FHF). Methods: We established experimental model of subacute hepatic failure.The immunohistochemical staining technique by NOS monoantibody was used. Results: The level of iNOS was correlated with ALT and TB level in the different phases after FS-112 administration and after operation,at the same time,iNOS level corresponded with liver histochemical change. Conclusion: NO involves in liver injury in experimental rats of fulminant hepatic failure induced by endotoxin.