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含内标的荧光PCR——荧光定量PCR 被引量:7

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摘要 1.前言 PCR技术应用于体外基因检测是临床诊断学上的一项重大突破,但由于传统的PCR检测易污染、假阳性率高等原因,被国家明令禁止使用于临床检验.为了克服传统PCR的上述缺点,发挥它早期、快速诊断的优点,近年来,发展了多种将传统PCR技术与其它技术结合起来的新型PCR技术,最成功的有PCR与ELISA结合的PCR-ELISA技术和PCR与荧光检测结合的荧光PCR技术.
出处 《中国医药导刊》 2001年第4期309-310,302,共3页 Chinese Journal of Medicinal Guide
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  • 2Ferre,F,Marchese,AL,Griffin,SL.Development and validation of a polymerase chain reaction method for the precise quantification of HIV-1 DNA in blood cells from subjects undergoing a 1-year immunotherapeutic treatment[].AIDS.1993
  • 3Ando, Y,H Kimure,H. Miwata, et al.Quantitative analysis of herpes simplex virus DNA in cerebrospinal fluid of children with herpes simplex encephalitis[].Journal of Medical Virology.1993

同被引文献49

  • 1施林祥,李东辉.实时荧光PCR研究新进展[J].世界华人消化杂志,2005,13(5):596-599. 被引量:22
  • 2高斌,刘敬忠.实时荧光PCR技术的研究及其应用进展[J].诊断学理论与实践,2005,4(6):507-509. 被引量:21
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