微粒子酶免法检测全血普乐可复浓度及其临床应用

Determination of the level of Tacrolimus in whole blood with MEIA and its clinical application

目的 评价微粒子酶免 (MEIA)法检测全血普乐可复浓度的准确性及其在器官移植术后抗排异治疗中的应用。方法 以MEIA法检测 6例心脏移植、2例肝脏移植、2例小肠移植和 7例肾脏移植患者不同时期全血中普乐可复的谷浓度 ,并结合临床情况调整普乐可复的用药剂量。以MEIA法测定高、中、低 3种标准普乐可复全血样品 ,求算MEIA方法学的回收率 ,分析MEIA的准确性及其对临床普乐可复用药的指导作用。结果 在临床 6 83个血样的 472次检测中 ,质控血样检测结果均在控制范围内 ,质控的平均RSD为 (7.7± 2 .0 ) % ,方法回收率为 (96 .4± 7.4) % ,分析过程可在 1h内完成。结论 MEIA法具有快速、简便、专一性强和灵敏度高的特点 ,是一种较理想的普乐可复血药浓度常规检测方法 。

Aim To evaluate the accuracy of detecting Tacrolimus concentration in whole blood by MEIA and its clinical application in organ transplantation. Methods The MEIA was used to detect Tacrolimus valley point concentrations in 6 heart transplanted,2 liver transplanted,2 small intestine transplanted and 7 kidney transplanted recipients at different postoperative periods. Based on the results combined with clinical findings, the dosage of Tacrolimus was adjusted. The recovery of the method was calculated by determining the level of Tacrolimus standard whole blood sample and guidance action in Tacrolimus clinical application was described. Results In 472 detections of 683 samples, the results of quality control samples were all in the control range. The RSD of control samples was (7.7±2.0)%. The recovery of the method was (96.4±7.4)%. Analysis course can be completed in 1 hour. Conclusion MEIA is a rapid, simple, specific and sensitive method for routine Tacrolimus concentration monitoring in whole blood, which is suitable for application in hospital.