圆锥角膜的免疫组织化学研究

Immunohistochemistry Research on Keratoconus

目的:了解圆锥角膜基底膜免疫组化的变化,以发现圆锥角膜可能的特异性改变,为探讨其发病机制提供依据。方法:收集圆锥角膜在我院眼科行穿透性角膜移植术的角膜片,用链霉亲和素-生物素法（SP法）检测圆锥角膜、角膜白斑、正常角膜基底膜IV型胶原、Fn、Ln表达的异同,并进行统计学分析。结果:圆锥角膜基底膜IV型胶原,Fn染色较正常角膜显著升高,但与角膜白斑比较无显著性差异。结论:圆锥角膜基底膜的改变可能与创伤愈合有关。提示圆锥角膜原发病变可能在角膜基质细胞。眼科学报2001;17:65～67。

Purpose: Keratoconus is a noninflammatory disease characterized by thining and ectasia of the central cornea. The etiology and pathogenesis of keratoconus remain unclear. We studied the composition of basement membranes (BM) in normal, scarred and kerat-conus corneas to find out possible changes specific for keratoconus. Methods: Paraffin-embeded sections of normal, scarred and keratoconus corneas were immunostained with antibodys against type IV collagen, Fibronectin (Fn) and Laminin (Ln).Results: Keratoconus had increased staining of the epithelial BM for a1-a2 chain of type IV collagen and Fn, especially in scarred corneal regions. A similar enhancement was also observed in scarred corneas, and there was no significant difference between keratoconus and scarred cornea.Conclusion: A process similar to wound healing would largely contribute to the differences seen in the keratoconus. Eye Science 2001; 17: 65 - 67.