RP-HPLC测定人血浆中还原型谷胱甘肽浓度

DETERMINATION OF GSH IN HUMAN PLASMA BY RP-HPLC

目的 :建立并考察人血浆中还原性谷胱甘肽的测定方法。方法 :采用邻苯二甲醛 (OPA)柱前衍生化RP-HPLC测定人血浆中谷胱甘肽的含量 ,并进行了方法学考察。结果 :3种浓度谷胱甘肽的平均回收率为 10 4.0 0 %± 3 .91% ,日内RSD≤ 3.6 9% ;日间RSD≤ 5 .38%。结论

OBJECTIVE:To establish a method for determination of GSH in human plasma by RP HPLC.METHODS:GSH was determined by OPA pre column derivatization RP HPLC.RESULTS:The recovery ratio was 104.00%±3.91%, RSD in day was 3.69%, RSD between days was 5.38%.CONCLUSION:This method was a good way to determined GSH in plasma.