髓鞘碱性蛋白微基因修饰雪旺细胞移植对大鼠脊髓损伤后生长相关蛋白-43表达的影响

Effect of microgene of pSVPoMcat to modify Schwann's cell on growth associated protein-43 expression after spinal cord injury in adult rats

目的 研究pSVPoMcat微基因修饰雪旺细胞 (Schwanncell,SC)对大鼠脊髓损伤 (spi nalcordinjury,SCI)后神经生长相关蛋白 - 4 3 (growthassociatedprotein - 4 3 ,GAP - 4 3 )表达的影响。 方法 采用 12 0只大鼠脊髓半横切损伤模型 ,随机分为三组 :pSVPoMcat微基因修饰SC移植组 (A组 )、单纯SC移植组 (B组 )、损伤对照组 (C组 )。应用免疫组织化学方法动态观察SCI后GAP - 4 3的表达 ,同时采用联合行为记分 (CBS)观察大鼠神经功能恢复情况。 结果 SCI损伤后 1周 ,A、B、C三组间GAP - 4 3表达差异无显著性意义 ;SCI后 2 ,4 ,8周 ,三组间表达顺序为A >B >C ,差异有显著性意义 (P <0 .0 5 ) ;其间A组在 2周时达到最高峰 ,此后逐渐下降 ,12周时A、B、C三组间差异无显著性意义。A组大鼠神经功能恢复最好。 结论 pSVPoMcat微基因修饰SC移植有促进SCI后GAP - 4

Objective To study the effect of microgene of pSVPoMcat implanted to modify Schwann's cells (SC)on growth associated protein 43 (GAP 43) expression after spinal cord injury in adult rats. Methods Hemisection of the T8 segment of the spinal cord was performed on all the experiment rats. The rats were randomly divided into three groups: Group A with microgene of pSVPoMcat implanted to genetically modify SC; Group B with SC implanted; Group C with hemisection of the spinal cord only. The changes of expression of GAP 43 in spinal cord were observed by immunochemistry with antibodies against GAP 43. Simultaneously, the combined behavorial score (CBS) was measured. Results There was not any significant difference ( P >0.05) among the three groups in the first week and the twelfth week. There was a significant difference ( P <0.05) among the three groups in the second, fourth, eighth week and more expression of GAP 43 at the second week in group A. The neurofunctional recovery was best in group A. Conclusions The microgene of pSVPoMcat implanted to modify SC can promote the expression of GAP 43 in spinal cord and functional recovery in adult rats after spinal cord injury.