细胞周期抑制蛋白p21^(cipl)在狼疮肾炎肾组织中的表达及其意义

Expression of cell cyclin inhibition protein p^(21cipl) in renal tissue of lupus nephritis and its significance

目的 了解狼疮肾炎（ＬＮ）中细胞周期抑制蛋白ｐ２１^(ｃｉｐｌ)的表达并探讨其与肾脏病病理改变、细胞增生及ＬＮ肾组织活动性的关系。方法 采用微波免疫组织化学染色法检测ＬＮ肾组织ｐ２１^(ｃｉｐｌ)表达，并进一步分析其与肾小球增殖细胞核抗原（ＰＣＮＡ）阳性细胞数、ＬＮ活动指数、细胞增生等肾脏病理改变的相关关系。结果 正常肾小球细胞无或仅有较弱 ｐ２１^(ｃｉｐｌ)。表达．ＬＮ肾小球细胞ｐ２１^(ｃｉｐｌ)的表达显著上调，尤以非Ⅳ型为显著。ＬＮ肾组织ｐ２１^(ｃｉｐｌ)阳性细胞数、ＰＣＮＡ阳性细胞数及ＬＮ肾组织活动指数显著负相关。结论 ｐ２１^(ｃｉｐｌ)在 ＬＮ肾小球细胞中呈高表达，且与肾小球细胞增生程度密切相关ｐ２１^(ｃｉｐｌ)可能参与ＬＮ发病的过程。

Objective To investigate the role of p^(21cipl) in the pathogenesis of lupus nephritis (LN), cell proliferation and its correlation with activity of LN. Methods Microwave-based immunohistochemistry was used to detect the expression of p^(21cipl) and PCNA in renal tissue of LN and normal control. At the same time , the correlation between expression of p^(21cipl) and renal injuries in LN was examined. Results p^(21cipl) was absent in the normal glomeruli. The expression of p^(21cipl) was significantly higher in LN than that in normal control. specially in non-type IV LN. The number of p^(21cipl) positive cells in renal tissue of LN showed a significantly negative correlation with PCNA protein expression and kidney tissue activity of LN. Conclusion Increased expression of p^(21cipl) in renal tissue of LN has a strong correlation with glomerular cell proliferation and may participate in the pathogenesis of lupus nephritis.