肝硬化大鼠肾脏水通道蛋白2和血管加压素V2受体的表达及黄芪对其的作用

Expression of renal aquaporin-2 and AVP-V2 receptor in liver cirrhosis and the role of astragalus memberanaceus

目的 观察肝硬化大鼠肾脏水通道蛋白 ２（ＡＱＰ２）和血管加压素（ＡＶＰ）－Ｖ２受体的表达情况，及中药黄芪对其的作用。方法 肾脏皮髓质 ＡＱＰ２ ｍＲＮＡ检测采用半定量 ＲＴ－ＰＣＲ法，肾脏ＡＱＰ２和ＡＶＰ－Ｖ２受体蛋白检测采用免疫组织化学法。结果 肝硬化 ２周和 ５周大鼠肾脏皮髓质ＡＱＰ２ ｍＲＮＡ、肾脏ＡＱＰ２和 ＡＶＰ－Ｖ２受体蛋白表达上调，黄芪在 ２周时可纠正这些指标的升高，５周时则不能。结论 肝硬化大鼠存在ＡＶＰ系统和ＡＱＰ２表达的异常，黄芪在早期对其有改善作用。

Objective To investigate the changes of AVP system and AQP2 in liver cirrhosis and the role of astragalus memberanaceus( AM) Methods Immunohistochemistry and relatively quantitative RT-PCR technique were used to determine the renal protein levels of AVP-V2 receptor and AQP2. and the renal levels of AQP2 mRNA of hepato cirrhotic rats induced by common bile duct ligation for 2 and 5 weeks. Results The hepato cirrhotic rats demonstrated obviously enhanced expression of AQP2 mRNA and protein, and V2 receptor protein in kidney. AM could alleviate the over expression of these parameters in compensated cirrhosis (2 weeks), but could not in decompensated cirrhosis (5 weeks) Condusion The abnormality of AVP system and AQP2 exists in hepato cirrhosis, which can be improved by AM in early stage.