果胶酶产生菌原生质体高能电子诱变效应

Studies of the Mutagaic effect of High-Energy Electron Irradiation on protoplasts of Paetinase Strain

目前果胶酶菌种选育多采用菌体诱变,本实验采用菌体的原生质体进行诱变,菌体原生质体经高能电子辐照(最适剂量为0.05×10~6rad),筛选出变异株16—1—1,此变异株生长发育旺盛,产酶性能显著优于亲株。经红外光谱分析,菌体孢子主吸收区为1125—1030cm^(-1),DNA分子主吸收区为1650—800cm^(-1),两者主吸收区极为相近,亲株原生质体经高能电子辐照后所产生的诱变株其红外吸收光谱表现出了某些峰值上的差异,DNA分子和果胶酶产生菌菌体孢子对红外吸收变化及诱变的相关性还有待进一步的研究。

Our experimental results indicated that the mutagnic effect of High-Electron Irradiation on protoplasts of pactinase strain Asp. 11-1-1 increased the growth, development and enzyme activity. Also reported that the wavetength of infrared absorption for pactinase strain Asp.11-1-1 is 800-1650 ( cm-1), and for DNA molecules, 1030-1125 ( cm-1 ). It seems that the wavetength of infrared absorption is very similar between Asp.11-1-1 and DNA. There are some changes on infrared absorption for DNA molecules of the mutant Asp.16-1-1 of High-Energy Electron Irradiation.